Please remember to include your OnCore tracking number with all correspondence and materials shipped to the facility.Submitting Samples for Standard (Single Tube) Sequencing Optimization methods can involve altering BigDye chemistry, changing thermocycling conditions, changing primers, or combining techniques. Our Full-Service option includes reprocessing if we feel that the initial sequencing yielded poor results or optimization of reaction conditions could potentially improve sequence length or quality. Pricing for this service includes either a Full Service or a No Redo option. Our standard Sanger sequencing allows for the highest flexibility in primer-template pairings and can better handle difficult templates or variations in DNA concentration. Please review our " fees for service" and " terms of service" pages before placing your order. Your samples can be sequenced using either facility stock primers, custom primers sent with your order, or custom primers synthesized at the facility. Our Sanger DNA sequencing services can process plasmid, PCR products, cosmids, phage, BAC, and bacterial genomic DNA samples in either a single tube or 96-well format. Sequencing on the AB 3730xl allows clients maximum flexibility in read length and number of samples. The AB 3730xl is a fully automated system that detects fluorescently labeled DNA fragments and can provide up to 800-1000 bases of high-quality DNA sequencing data per reaction. Both standard and high throughput Sanger DNA sequencing samples are run on the facility's two Applied Biosystems 3730xl DNA Analyzer. The DNA Facility's Sanger DNA sequencing services are optimized for the rapid turnaround of high-quality sequence data for individual samples or high-throughput plate sequencing. Coordinating Sequencing & Oligo Synthesis.
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